PIRA PCR designer for restriction analysis of single nucleotide polymorphisms

UNLABELLED Primer-introduced restriction analysis (PIRA-PCR) is widely used to detect Single Nucleotide Polymorphisms (SNPs). To create artificial Restriction Fragment Length Polymorphism (RFLP), a mismatch is usually introduced near the end of the primer that is close to the mutation of interest. We describe in this report a www-based computer program that screens for the suitable mismatches, designs the primers, lists the appropriate restriction enzymes and other related information. AVAILABILITY The computer program, with related descriptions, is available at http://cedar.genetics.soton.ac.uk/public_html/primer2.html.